| dc.description.abstract | Campylobacter spp. are a major cause of human gastroenteritis. Their wide host range, environmental
distribution and high genetic diversity contribute to the complex molecular epidemiology
of campylobacteriosis. The aim of this multidisciplinary thesis is to investigate the
phenotype:genotype relationships of C. jejuni and how they in
uence the micro-evolution of
these bacteria in New Zealand.
The first study used a time series of genotyped human campylobacteriosis cases from a region
of New Zealand to investigate if the clonal complexes (CCs) identified in human cases showed
a seasonal pattern. The analysis revealed a prevalent clonal complex (CC-45) which showed a
consistent summer peak.
The second study applied phylogenetic and population genetic tools to describe the population
structure and host associated genotypes within the C. jejuni population in wild and agricultural
animals. The findings showed that the C. jejuni isolates from non-agricultural animals exhibit a
higher number of mosaic alleles and fewer shared sequence types (STs) between the host groups,
whereas the C. jeuni in agricultural animals show a higher number of shared STs and fewer
occurrences of admixture.
The third study tested the ability of a variety of C. jejuni isolates to utilise 95 substrates
as carbon sources and tested their tolerance to different osmotic conditions using phenotypic
microarray (PM) technology. These phenotypic expressions were correlated with their genomes
and a genome wide association study was used to identify genes associated with the observed
phenotype.
The last study made use of data from a dual isolate chicken challenge. The study showed
the out-competition of one challenge strain and genetic variations of 15 core single nucleotide
polymorphisms (SNPs), 14 of which were non-synonymous point mutations. These SNPs were
confined to nine genes all of which were associated with cell shape, chemotaxis or motility of the
bacteria.
This thesis has furthered our understanding of the seasonality of human campylobacteriosis in
New Zealand, the existing population structure of C. jejuni, its biochemical requirements and
tolerance to osmolytes and novel insights into short-term evolutionary dynamics in vivo. Based
on these findings and the recommendations for future directions, this could lead to a greater
understanding of host-association and new intervention strategies. | en_US |
